1.SOAPDenovo配置文件示例
软件下载安装和使用:http://soap.genomics.org.cn/soapdenovo.html
asm.cfg
#maximal read length
max_rd_len= [LIB]
avg_ins=450 #if sequence needs to be reversed
reverse_seq=0 #in which part(s) the reads are used
asm_flags=3 #use only first 100 bps of each read
rd_len_cutoff=100 #in which order the reads are used while scaffolding
rank=1 # cutoff of pair number for a reliable connection (at least 3 for short insert size)
pair_num_cutoff=
#minimum aligned length to contigs for a reliable read location (at least 32 for short insert size)
map_len=32
#a pair of fastq file, read 1 file should always be followed by read 2 file
q1=../../data/newBGIseq500_1.fq.gz
q2=../../data/newBGIseq500_2.fq.gz
运行脚本:
run.sh
/home/stu2/Software/Assemblathon1_pipeline/SOAPdenovo-63mer_v2. all -s asm.cfg -K -p -R -o asm >cout.log >cerr.log
./SOAPdenovo-63mer_v2. pregraph -K -s asm.cfg -o asm -p >pregraph.log >pregraph.err
./SOAPdenovo-63mer_v2. contig -s asm.cfg -g asm -M -e -p -R -D >contig.log >contig.err
./SOAPdenovo-63mer_v2. map -s asm.cfg -g asm -k -p >map.log >map.err
./SOAPdenovo-63mer_v2. scaff -g asm -p -F >scaff.log >scaff.err
02. VCFtools的使用
软件下载和使用:
https://vcftools.github.io/documentation.html
https://vcftools.github.io/man_latest.html
# get Qual
./vcftools --gzvcf chr17.vcf.gz --site-quality --out Qual
# get interval
./vcftools --gzvcf chr17.vcf.gz --chr chr17 --from-bp --to-bp --remove-indels --out TP53 --recode
03.变异位点信息统计
#!/usr/bin/perl
use strict; my $file = shift; open(In,"gzip -dc $file|") or die ("can't open the file!\n"); my @type;
my @array = ( );
while(<In>){
chomp;
next if (/^##/);
if (/^#/){
my @line = split;
push @type, $line[];
push @type, $line[];
push @type, $line[];
next;
}
my @line = split;
if ($line[] >= && $line[] <= && $line[] ne "."){
my @type1 = split(/\/|:/, $line[]);
my @type2 = split(/\/|:/, $line[]);
my @type3 = split(/\/|:/, $line[]);
#print "$type1[0]\t$type1[1]\n";
if ($type1[]==$type1[]){
$array[][] ++;
}else{
$array[][] ++;
}
if ($type2[] == $type2[]){
$array[][] ++;
}else{
$array[][] ++;
}
if($type3[] == $type3[]){
$array[][] ++;
}else{
$array[][] ++;
}
}
}
close IN; print "Sample\tHomozygous\tHeterozygote\n";
print "$type[0]: $array[0][0]\t$array[0][1]\n";
print "$type[1]: $array[1][0]\t$array[1][1]\n";
print "$type[2]: $array[2][0]\t$array[2][1]\n";
04. KmerFreq使用脚本
工具包地址:ftp://public.genomics.org.cn/BGI/SOAPdenovo2
kmer.sh
./kmerfreq -k -l lib.list -t >17mer.freq >17mer.log
lib.list
reads1.fq.gz
reads2.fq.gz
友情参考链接:http://20xue.com/3997.html,https://www.cnblogs.com/azrael-cc/