PacBio软件总览 - 初级分析

时间:2022-03-18 19:06:27

PacBio® RS Software Overview

  • PacBio运行的整个流程是什么?每一步都用到了什么软件?

PacBio软件总览 - 初级分析

PacBio软件套件

PacBio软件总览 - 初级分析

RS Remote:Design runs remotely,Can assign multiple SMRT® Cells per well with different movie times

RS Touch:Loading a Run,Monitor at the instrument or remotely,View real-time base incorporations,Displays remaining run time,Shows SMRT® Cell status, from cell prep to Quality Values

PacBio® RS:测序仪

Primary Analysis Pipeline:

PacBio软件总览 - 初级分析

SMRT® Analysis – Secondary Analysis:

SMRT Portal:Job Selection(Prior jobs are searchable,Can select one or more jobs),Report Viewing()

SMRT View:Genome Browser,View Base Modification Details

PacBio软件总览 - 初级分析


Primary Analysis

  • PacBio测序仪的光学原理,如何得到的fastq文件
  • 测序结果都有哪些文件,各代表什么意思?

PacBio的光学检测系统:

PacBio软件总览 - 初级分析

Algorithms reduce photon counts vs. time to sequences in real time